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KMID : 0043319840070010011
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Archives of Pharmacal Research
1984 Volume.7 No. 1 p.11 ~ p.15
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Drug-Biomacromolecule Interaction VII -Binding of Six Cephalosporins to Human Serum Albumin Using Fluorescence Probe Technique and Difference Spectra-
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Kim CK
Yang JS/Lim YS
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Abstract
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Binding of six cephalosporins (cefotaxime, cefuroxime, cefazoline, cephalothin, cephaloridine, cephacetrile) to human serum albumin was studied. Fluorescence probe technique and difference spectrophotometry were employed to evaluate the nature and degree of association of cephalosporin-albumin complex. 1-anilinonaphthalene-8-sulfonate was used as the fluorescence probe, and 2-(4"-hydroxybenzeneazo) benzoic acid as the UV spectrophotometric probe. Competitive bindings between cephalosporins and probe were observed. For the binding of cephalosporins to human serum albumin, three binding sites were identified by fluorescence probe technique but four binding sites by UV spectrophotometry. The binding constants of cephalosporins to human serum albumin measured by fluorescence probe technique are higher than those measured by UV spectrophotometry.
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